Midgut-specific immune molecules are produced by the blood-sucking insect Stomoxys calcitrans
نویسندگان
چکیده
منابع مشابه
Midgut-specific immune molecules are produced by the blood-sucking insect Stomoxys calcitrans.
We have cloned and sequenced two defensins, Smd1 and Smd2, from anterior midgut tissue of the blood-sucking fly Stomoxys calcitrans. The DNA and N-terminal protein sequences suggest both are produced as prepropeptides. Smd1 differs from the classic defensin pattern in having an unusual six-amino acid-long N-terminal sequence. Both Smd1 and Smd2 have lower pI points and charge than insect defens...
متن کاملAssociation of midgut defensin with a novel serine protease in the blood-sucking fly Stomoxys calcitrans.
Using ELISA we provide direct evidence that the midgut defensins of the blood-sucking fly Stomoxys calcitrans are secreted into the gut lumen. We show that midgut defensin peptide levels increase up to fortyfold in response to a blood meal but not to a sugar meal. The data suggests the midgut defensin genes are post-transcriptionally regulated and that their function is protection of the stored...
متن کاملEpithelial innate immunity. A novel antimicrobial peptide with antiparasitic activity in the blood-sucking insect Stomoxys calcitrans.
The gut epithelium is an essential interface in insects that transmit parasites. We investigated the role that local innate immunity might have on vector competence, taking Stomoxys calcitrans as a model. S. calcitrans is sympatric with tsetse flies, feeds on many of the same vertebrate hosts, and is thus regularly exposed to the trypanosomes that cause African sleeping sickness and nagana. Des...
متن کاملIsolation of Enterobacter sakazakii from Midgut of Stomoxys calcitrans
To the Editor: Enterobacter saka-zakii, a gram-negative rod-shaped bacterium, is an emerging foodborne pathogen that can cause meningitis, sepsis, or necrotizing enterocolitis in newborns, particularly affecting premature or other immunocompromised infants. Although an environmental reservoir and mode of transmission for E. sakazakii has not been clearly identified, a growing number of reports ...
متن کاملHaemozoin formation in the midgut of the blood-sucking insect Rhodnius prolixus.
Malaria parasites digest haemoglobin and detoxify the free haem by its sequestration into an insoluble dark-brown pigment known as haemozoin (Hz). Until recently, this pigment could be found only in Plasmodium parasites. However, we have shown that Hz is also present in the midgut of the blood-sucking insect Rhodnius prolixus [Oliveira et al. (1999) Nature 400, 517-518]. Here we show that Hz sy...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1997
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.94.21.11502